Lou AAA

Medical Data AnalystFull Time

• Job Details:Researching current medical literature, curating, and building up-to-date reliable objective medical data framework Building & standardizing parameters to extract the most critically relevant short and long term morbidity and mortality risk elements from health record files of diverse-source With ultimate goal: simplify, accelerate, and optimize the underwriting process of disability & life insurance Excel: Advanced skills including Visual Basic macros, searching, sorting, matching, text extraction/editing

Research AssociateFull Time

• Job Details:​​Pilot project on computationally predicting the cellular response to drug combinations (inspired by Connectivity Map) Wrote the protocol & implemented independently all laboratory stages including ordering cost-effective supplies, literature search, initiating, maintaining and storing cell culture Cultured mammalian immortal cells (MCF7) using aseptic/sterile techniques, created frozen cell stock Established optimum drug concentration to maximize mRNA cellular response Treated cells with drugs, homogenized cells, isolated & quantified RNA (spectrophotometry) Submitted weekly reports to my PI on the results, data analysis/graphing, my interpretation, troubleshooting to overcome challenges, made suggestions for future protocol optimization

Process Development Associate Full Time

• Job Details:Sequencing gaps and difficult-to-sequence regions in different genomes Used Primer Walking method to sequence gaps Programmed & operating Multiprobe robot for automatic liquid reagents dispensing Routinely used 96-wells, made labels, barcode plate reader, thermal plate sealer Used Standard Operating Procedures (SOP) in daily robot maintenance & troubleshooting